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KMID : 1134820170460050646
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Journal of the Korean Society of Food Science and Nutrition
2017 Volume.46 No. 5 p.646 ~ p.652
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Method Validation for Determination of Lignan Content in Fermented Sesame by Bioconversion
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Jung Tae-Dong
Kim Jae-Min
Choi Sun-Il
Choi Seung-Hyun
Cho Bong-Yeon
Lee Jin-Ha
Lee Sang-Jong
Park Seon-Ju
Heo In-Young
Lee Ok-Hwan
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Abstract
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The aim of this study was to investigate method validation for determination of sesamol, sesamin, and sesamolin in non-fermented sesame and fermented sesame by bioconversion. For validation, the specificity, linearity, precision, accuracy, limits of detection (LOD), and quantification (LOQ) of sesamol, sesamin, and sesamolin were measured by HPLC. Linearity tests showed that the coefficients of calibration correlation (R2) for sesamol, sesamin, and sesamolin were 0.9999. Recovery rates of lignan contents in non-fermented and fermented sesame were high in the ranges of 100.27∼115.10% and 98.43∼114.90%, respectively. The inter-day and intra-day precisions of sesamin and sesamolin analyses for non-fermented and fermented sesame were 0.27∼1.94% and 0.25∼0.69%, respectively. The LOD and LOQ were 0.23∼0.34 μg/g and 0.70∼1.03 μg/g, respectively. These results indicate that the validated method is appropriate for the determination of sesamol, sesamin, and sesamolin.
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KEYWORD
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Sesamum indicum L., lignan, method validation, bioconversion, HPLC-PDA
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